张建林，邬开朗，张 雪，朱 应，李 雁，赵伟光**，吴建国( 武汉大学生命科学院病毒学教育部重点实验室，湖北武汉 430072 )Construction and Expression of Vectors Expressing siRNAZHANG Jian-lin，WU Kai-lang，ZHANG Xue，ZHU Ying，Li Yan，ZHAO Wei-guang**，WU Jian-guo(College of Life Sciences, Wuhan University/Key Laboratory of the Ministry of Education for Virology, Wuhan 430072, Chin...

Charles Addo-Quaye,1 Tifani W. Eshoo,2 David P. Bartel,4,5and Michael J. Axtell2,3,*1Department of Computer Science and Engineering2Cell and Developmental Biology Graduate ProgramHuck Institutes of the Life Sciences3Department of BiologyPennsylvania State UniversityUniversity Park, Pennsylvania 168024Whitehead InstituteCambridge, Massachusetts 021425Howard Hughes Medical In...

In contrast to microRNAs and Piwi-associated RNAs, short interfering RNAs (siRNAs) are seemingly dispensable for host-directed gene regulation in Drosophila. This notion is based on the fact that mutants lacking the core siRNA-generating enzyme Dicer-2 or the predominant siRNA effector Argonaute 2 are viable, fertile and of relatively normal morphology1,2. Moreover, endogen...

A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands Alexander Stark, Natascha Bushati, Calvin H. Jan, Pouya Kheradpour, Emily Hodges, Julius Brennecke,David P. Bartel, Stephen M. Cohen and Manolis Kellis MicroRNAs (miRNAs) are ∼22-nucleotide RNAs that are mprocessed from characteristic precursor hairpins and pair to sites in messag...

Improved polymerase ribozyme efficiency on hydrophobic assemblies ULRICH F. MU¨ LLER1 and DAVID P. BARTELWhitehead Institute for Biomedical Research, Department of Biology, and Howard Hughes Medical Institute, Massachusetts Instituteof Technology, Cambridge, Massachusetts 02142, USAABSTRACTDuring an early step in the evolution of life, RNA served both as genome and as ...

Total RNA extraction from Arabidopsis and tobacco Allison C. Mallory, Bartel Lab Whitehead Institute

dsRNA Soaking Materials:1) T25 or T75 tissue culture flasks 2) 6-well tissue culture plates 3) 15-ml Corning conical tubes 4) Normal S2 complete medium (S2 containing 10% serum) 5) S2 medium containing 2%, and15% serum 6) SFM medium 7) Trypan Blue (GIBCO, catalog number: 15250-061)

Materials:6-well plates or 3cm dishes 15ml conical tubes sterile Eppendorf tubes FuGENE 6 Transfection Reagent* (Roche, Cat # 1 814 443)* FuGENE 6 is a multi-component lipid-based transfection reagent that complexes with and transports DNA into the cell during transfection.

曹国军，邵宁生*(军事医学科学院基础医学研究所， 北京100850)摘　要：RNA 技术可以分为RNA 基础研究相关的技术、RNA 应用相关的技术和RNA 的生物信息学技术。RNA 基础研究相关技术包括RNA 分离纯化和鉴定技术、RNA 与其他生物大分子相互作用技术、RNA 高级结构的研究技术和其他相关RNA 技术；RNA 应用相关技术则包括用于生产其他产品的RNA技术和直接用于药物开发的RNA 技术；RNA 的生物信息学技术则有各种...

张筱晨， 周 惠， 屈良鹄*(中山大学生物工程研究中心，广州510275)摘　要：核仁小分子RNA(snoRNA)是一类广泛分布于真核生物细胞核仁的小分子非编码RNA，具有保守的结构元件，并以此划分为3 大类：box C/D snoRNA、box H/ACA snoRNA 和MRP RNA。其中box C/D 和box H/ACA 是已知snoRNA 的主要类型，以碱基配对的方式分别指导着核糖体RNA 的甲基化和假尿嘧啶化修饰。研究发现，snoRNA 除了在核糖体RNA 的生物...

摘要：RNA沉默在机体防御病毒入侵和调控基因表达中发挥着重要的作用，目前已成为一种有效的工具应用于基因功能研究和疾病治疗等领域．RNA沉默现象普遍存在于真核细胞中，然而在宿主与病毒漫长的进化过程中，病毒已经演化出一系列逃逸或抑制RNA沉默作用的方法和途径，使RNA沉默效果显著降低，另一方面，哺乳动物体细胞自身也存在调节RNA沉默功能从而使生命活动的调节更加精细完善.为了使RNA沉默发挥它的潜在...

刘默芳*，王恩多(中国科学院上海生命科学研究院生物化学与细胞生物学研究所，分子生物学国家重点实验室，上海200031) 摘　要：小分子调控RNA，包括siRNA (small interfering RNA)、miRNA (microRNA)和piRNA (piwi interacting RNA)、hsRNA (heterochromatin associated small RNA)等，是当前生命科学研究的前沿热点。越来越多的证据表明，这些小分子RNA 存在于几乎所有较高等的真核生物细胞中，对生物体具...

分子植物育种，2006 年，第4 卷，第6(S)期，第83-90 页Molecular Plant Breeding, 2006, Vol.4, No.6(S), 83-90 卢东长城1,2 梁荣奇2*1 首都师范大学生命科学学院, 北京, 100037; 2 北京市农林科学院北京农业生物技术研究中心, 北京, 100097* 通讯作者, liangrq@hotmail.com摘要Fire 等(1998)发现在线虫中双链RNA 可以有效地阻断同源基因的表达，并将这一现象称为RNA干扰(RNA interference, RNAi)，从而获...

Constance L. Cepko *Elizabeth Ryder1Christopher Austin2Jeffrey Golden3Shawn Fields-Berry *John Lin * * Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute, Boston, MA 02115 1 Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA 01609 2 Merck Research Laboratories, West Point, PA 19486 3 Department of Pathol...

RNA Whole Mount In Situ Hybridization Core protocol for both mouse and chick embryos Cepko/Tabin lab Fix embryos in fresh 4% paraformaldehyde (or a thawed frozen aliquot) in PBS at 4° C overnight. Dissect embryos (as needed) into PBT, and wash once in PBT for 5 minutes with gentle rocking. Dehydrate embryos into methanol using a graded metha...

2 Introduction: SCIENCE RNAiKeith Jolliff3 Introduction:The RNAiWorldGuy Riddihough4 A Species of Small Antisense RNA inPosttranscriptional Gene Silencing in PlantsAndrew J. Hamilton and David C. BaulcombeScience 29 October 1999 286: 950–9528 Argonaute2 Is the Catalytic Engine of Mammalian RNAiJidong Liu, Michelle A. Carmell, Fabiola V. Rivas, Carolyn G. Marsden,J. Mi...

This year’s Nobel Prize in Physiology or Medicine is shared by Professor Andrew Z. Fire at Stanford University, California, USA, and Professor Craig C. Mello at the University of Massachusetts Medical School in Worcester, USA. They receive the prize for their discovery that double-stranded RNA triggers suppression of gene activity in a homology-dependent manner, a pro...

Iain D.C. FraserAlliance for Cell Signaling, Molecular Biology LaboratoryCalifornia Institute of Technology, Pasadena, CA IntroductionRNA interference (RNAi) has recently emerged as a powerful experimental tool in mammalian cell biology. Double-stranded RNA (dsRNA)-induced gene silencing was originally discovered as a mechanism for regulating expression of endogenous genes...

Its wonderful to be here today, I would like to start with the most important part, by saying thank you. First of all, I want to thank Andy Fire for being such a tremendous colleague, friend, and collaborator going back over the years. Without Andy I definitely wouldnt be here today. I need to thank the University of Massachusetts for providing for my laboratory, for beli...

张松莲 ，一， 曾富华 ， ，喻宁华 ，一，饶力群2(1．湛江师范学院应用生命科学研究所，广东湛汀524048；2．湖南农业大学生物科学与技术学院，长沙410128)摘要：miRNAs是真核生物中的一类5’端带磷酸基团、3’端带羟基、长度在22 nt左右的内源性非编码调控RNAs。miRNAs在控制植物的发育、开花时序、新陈代谢、应激反应等方面起着重要的作用。已知植物miRNAs在转录后水平上抑制基因表达，主要是通...