内容简介:  

Real Time PCR

A useful new approach?

 Statistical Problems?

Reverse transcription followed by Polymerase Chain Reaction Considered to be the most sensitive method for the detection and quantification of gene expression levels. Used as a follow-up when a particular gene is suggested in micro-array studies. Potential problems with sensitivity, specificity and reproducibility.

 

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• · Real-Time PCR in Microbiology
• · Real-Time Primer Design for DNA Chips
• · Introduction to Real-Time PCR
• · GMO specific real-time PCR system
• · Critical factors for success in real-time, multiplex PCR
• · Real-Time PCR Vs. Traditional PCR
• · DNA Quantitation and Real-Time PCR
• · Why is Real Time PCR Important?
• · A novel quantitative real-time PCR test for Mycobacterium tuberculosis
• · Quantitative real-time PCR protocol for analysis