RFLP
Stands for Restriction Fragment Length Polymorphism
Takes advantage of differences in DNA between individuals that result in different fragments when digested with restriction enzymes
RFLP
To see RFLP, DNA is digested with the appropriate restriction enzymes and run on an agarose gel.
A Southern Blot is performed to complete the analysis.
Southern Blotting
A method to visualize specific segments of DNA– usually a particular gene.
Uses radioactive probes that bind to the specific DNA segments
Ex. When testing for the hemoglobin alleles, the probe would bind to these regions
Southern Blotting
Steps:
Soak gel in basic solution to separate DNA strands
Transfer DNA on to a nylon membrane (spacing of DNA is maintained)
Incubate with radioactive probe for specific segment
Wash away unbound probe
Detect probes using x-ray film autoradiograph