Introduction to Real-Time PCR
Henrik Ericson
Applied biotechnology
November, 2007

Introduction to Real-Time PCR

PCR
Theory and applications ofReal-Time PCR
Understanding Real-Time PCR fluorescence
Real-Time PCR assays
Optimization of Real-Time PCR
Working with RNA

• · Real-Time PCR in Microbiology
• · Real-Time PCR
• · Real-Time Primer Design for DNA Chips
• · GMO specific real-time PCR system
• · Critical factors for success in real-time, multiplex PCR
• · Real-Time PCR Vs. Traditional PCR
• · DNA Quantitation and Real-Time PCR
• · Why is Real Time PCR Important?
• · A novel quantitative real-time PCR test for Mycobacterium tuberculosis
• · Quantitative real-time PCR protocol for analysis