dsRNA Soaking

Materials:
1) T25 or T75 tissue culture flasks
2) 6-well tissue culture plates
3) 15-ml Corning conical tubes
4) Normal S2 complete medium (S2 containing 10% serum)
5) S2 medium containing 2%, and15% serum
6) SFM medium
7) Trypan Blue (GIBCO, catalog number: 15250-061)