内容简介:  

Description
This tutorial will discuss the evolution of traditional PCR methods towards the use of Real-Time chemistry and instrumentation for accurate quantitation.
Objectives
This tutorial will provide an understanding of the following:

Limitations of traditional PCR

Introduction to Real-Time PCR

Advantages of Real-Time chemistries over traditional PCR
methods

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• · Real-Time PCR in Microbiology
• · Real-Time PCR
• · Real-Time Primer Design for DNA Chips
• · Introduction to Real-Time PCR
• · GMO specific real-time PCR system
• · Critical factors for success in real-time, multiplex PCR
• · DNA Quantitation and Real-Time PCR
• · Why is Real Time PCR Important?
• · A novel quantitative real-time PCR test for Mycobacterium tuberculosis
• · Quantitative real-time PCR protocol for analysis