内容简介:  

Eric Lader, Ph.D
elader@ambion.com
Marketing Product Manager
Manager, Product
Applications Group

Agenda
Sample prep considerations
• Tissue handling : do’s and don’ts
• RNA isolation: what’s important
• My favorite RNA isolation method
• Genomic DNA contamination
RT considerations you’ve never considered
• Endogenous priming
• RT inhibition of PCR
• RT reaction times

Isolating RNA: Issues and Concerns
Performance
suitability for downstream applications

 

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Yield
rapid tissue preservation
rapid and thorough sample disruption
Quality
intactness: rapid sample preservation and disruption
purity: removal of contaminants and genomic DNA
stability: complete inactivation of RNase

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• · Real-Time PCR in Microbiology
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• · Real-Time Primer Design for DNA Chips
• · Introduction to Real-Time PCR
• · ALKALINE LYSIS DNA PREP (E. COLI)
• · GMO specific real-time PCR system
• · Critical factors for success in real-time, multiplex PCR
• · Real-Time PCR Vs. Traditional PCR
• · DNA Quantitation and Real-Time PCR
• · Why is Real Time PCR Important?
• · A novel quantitative real-time PCR test for Mycobacterium tuberculosis