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• Before any particular protein can be sequenced and characterized, its is necessary to separate it from all of the other proteins in the cell.
• This “purification” process involves separating proteins based on their ionic properties, their sizes, their hydrophobicity, and their affinities for certain molecules (ligands). Each successive step is referred to as fractionation.
• Typically some form of column chromatography is employed, in which the solid phase (stationary phase) contains molecules that in some way exploit t

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