内容简介:  

Introduction
The RT-PCR kit contains polymerase and all necessary reagents to perform single tube RT and PCR. However, we will break the reaction into two discrete steps, RT (reverse transcription to convert mRNA to cDNA) and PCR (to amplify cDNA by the polymerase chain reaction). The RT reaction involves synthesizing cDNA from total RNA or polyA enriched RNA (mRNA). This cDNA is now ready to amplify by PCR. Using specific oligonucleotides (i.e. primers) a fragment of the cDNA can be amplified.

下载地址:         下载地址