EGFP assay

EGFP will be measured by direct visualisation with a UV microscope and the appropriate filters. By using an inverted microscope, we are able to assay the cells directly whilst they are still alive. The use of GFP (and its variants) has been a very powerful addition to the molecular biologist in recent years as real time experiments can be performed.
We will measure the percentage of cells expressing EGFP by physically counting several fields of view on the microscope. Although this is maybe not the most accurate method, it gives some idea of the transfection efficiency.