侯丙凯1 ,2 ,夏光敏1 ,陈正华2
(11 山东大学生命科学学院,济南250100 ;21 中国科学院遗传研究所,北京100101)
摘 要:外源基因在转基因植物中表达效率低一直是令相关研究者困扰的一个问题。转基因植物的生物安全性最近在全球范围内开始引起世人的担忧。本文简要介绍了近年来在植物表达载体构建方面所采用的一些新策略,这些策略有助于增强外源基因的表达水平、提高生物工程体的安全性。
关键词:植物;转基因;表达载体;优化
Strategies for Optimizing Expression Vectors Used in Plant Genetic Engineering
HOU Bing2kai1 ,2 ,XIA Guang2min1 ,CHEN Zheng2hua2
(1. College of L if e Sciences , S handong University , Ji nan 250100 , Chi na ;
2. Instit ute of Genetics , Chi nese Academy of Sciences , Beiji ng 100101 , Chi na)
Abstract :The low expression level of foreign genes in transgenic plants is a puzzling question for researchers in plant genetic engineering. The biosafety concern is now causing a growing public wariness of transgenic plants around the world. This article reviews the new strategies currently used in construction of plant expression vector for plant genetic engineering.These strategies are important for obtaining better expression of foreign genes and developing safer transgenic plants.
Key words :plant ;transgene ;expression vector ;optimization
